完整的透化肌原纤维测试系统

温度调节组织浴

简单安装在倒置显微镜上

计算机控制的电动XYZ微定位平台

包括缓冲阀和解决方案切换器控制器

控制和测量力，长度和肌节间距

测量力-pCa，kTr，刚度，长度 - 张力和力 - 速度关系

与Aurora Scientific 900B高速视频Sarcomere长度系统（HVSL）无缝连接

参考文献：

Schappacher-Tilp, Gudrun et al. “Computing Average Passive Forces in Sarcomeres in Length-Ramp Simulations .” PLoS Computational Biology 12.6 (2016): e1004904.

Cornachione, Anabelle S. et al. “The increase in non-cross-bridge forces after stretch of activated striated muscle is related to titin isoforms.” American Journal of Physiology - Cell Physiology 310.1 (2016): C19-C26.

Leite, Felipe S. et al. “Reduced passive force in skeletal muscles lacking protein arginylation.” American Journal of Physiology - Cell Physiology 310.2 (2016): C127-135.

Powers, Krysta et al. “Decreased force enhancement in skeletal muscle sarcomeres with a deletion in titin.” Journal of Experimental Biology 219.9 (2016): 1311-1316.

Minozzo, Fábio C., David Altman, and Dilson E. Rassier. “MgADP activation contributes to force enhancement during fast stretch of isolated skeletal myofibrils.” Biochemical and Biophysical Research Communications 463.4 (2015): 1129-1134.

Philippou, Anastassios et al. “Masticatory muscles of mouse do not undergo atrophy in space.” The FASEB Journal 29.7 (2015): 2769-2779.

Rassier, Dilson, and Aleksander Labuda. “Method and apparatus for measuring cantilever deflection in constrained spaces.” (2014): U.S. Patent No. 8,667,611.

Cornachione, Anabelle S. et al. “Arginylation of Myosin Heavy Chain Regulates Skeletal Muscle Strength.” Cell Reports 8.2 (2014): 470-476.

Yamada, Takashi et al. “Nitrosative modifications of the Ca2+ release complex and actin underlie arthritis-induced muscle weakness.” Annals of the Rheumatic Diseases 74.10 (2014): 1907-1914.

Ribeiro, Paula A.B. et al. “Contractility of myofibrils from the heart and diaphragm muscles measured with atomic force cantilevers: Effects of heart-specific deletion of arginyl-tRNA-protein transferase.” International Journal of Cardiology 168.4 (2013): 3564-3571.

Labuda, Aleksander et al. “Optical detection system for probing cantilever deflections parallel to a sample surface.” Review of Scientific Instruments 82.1 (2011): 013701.

Rassier, Dilson E. “Striated Muscles: From Molecules to Cells.” Advances in Experimental Medicine and Biology Muscle Biophysics (2010): 1-6.

Joumaa, Venus, Walter Herzog, and Dilson E. Rassier. “904-Plat Critical Sarcomere Length Extension and Phase Transition Of Force During Lengthening Of Skeletal Muscle Myofibrils.” Biophysical Journal 94.2 Supplement (2008): 305-307.

1700A肌原纤维系统是第***个商业化的肌原纤维系统，专门用于测量单个肌原纤维激活产生的纳米牛顿力。这是***个完整的测试系统，包括表征肌原纤维收缩所需的所有硬件。1700A包括：470A肌原纤维原子力显微镜（AFM）传感器，340A压电长度控制器，温控浴槽，470A-XYZ电动平移台，820A双XYZ运动控制器，用于计算机控制AFM悬臂和玻璃针定位，溶液阀控制器，解决方案切换电机驱动器和我们的实时Linux 600A数据采集系统。

470 Myofibril AFM传感器利用原子力显微镜和光束偏转方法测量单个肌原纤维激活产生的纳米牛顿力。肌原纤维固定在AFM悬臂和玻璃针之间，AFM悬臂用作高灵敏度力传感器，玻璃针连接到340A压电长度控制器。控制长度和测量力使1700A肌原纤维系统能够提供肌原纤维的完整机械表征。根据所使用的悬臂，满量程力为1-10μN，惊人的分辨率为1nN。

1700A包含温度调节组织浴以及缓冲液交换系统的控制，这是快速激活任何肌原纤维的关键组分。该设备可以很容易地安装在倒置显微镜上，为浴缸和肌原纤维提供方便和清晰的通道。

用于肌原纤维机械表征的可定制方案可以使用我们的600A数据采集系统进行，当与我们的可选900B / 901C VSL肌节长度测量软件结合使用时，研究人员可以控制和测量长度，力和肌节长度。