美国Orbigen
Orbigen位于风景如画的美国加州SAN DIEGO,其主要成员来自该地区著名的生物科技公司和大学,在真核表达,逆转录表达,SiRNA,抗体开发等方面居于世界领先地位。著名品牌有:
Sapphire Baculovirus真核表达系统及预制基因库
Orbigen公司可以提供几百种重组的杆状病毒表达载体,其涉及的外源基因主要来自于人、大鼠、小鼠、酵母、病毒等多种生物。除提供插入单个外源基因的重组杆状病毒表达载体外,该公司还可以提供多种插入GST、His6标记融合基因的重组杆状病毒表达载体,极大地方便了客户的科研工作。
Phoenix Retrovirus逆转录表达系统
该系统应用非常广泛,目前世界上有近2500家实验室采用该系统转染将外源基因转入分化的哺乳动物细胞。采用LacZ和GFP基因作为报告系统。
试剂盒组分:
* PhoenixTM ampho 包装细胞
* PhoenixTM eco 包装细胞
* 10 μg pBMNZ 质粒
* 10 μg pBMN-GFP 质粒
BaculoBooster Virus Kit
该试剂盒包括10种不同的杆状病毒库,每个杆状病毒库都含有表达一组真核生物伴侣蛋白的重组杆状病毒混合物,这些伴侣蛋白可以在感染的昆虫细胞(Sf9, Sf21 and T.ni)知道重组蛋白的折叠。该杆状病毒库可以与感染细胞内的重组杆状病毒相互作用,优化目的蛋白的表达水平和折叠。
pCMV1/2/3 Xi-Clone 和pIX1/2/3 Xi-Clone 克隆表达系统
pCMV1/2/3 Xi-Clone?系统有3种不同的类型,每种类型分别包含一种pCMV载体,可以快速高效地实现体内定向克隆。这类pCMV载体含有高度优化的细胞巨化病毒(CMV)的启动子/增强子序列,因此表达效率比其它的CMV类载体(如:pcDNA3)要高好几倍。
pIX1/2/3 Xi-Clone?含有有3种不同的类型(pIX、pIX2和pIX3),每种类型分别包含一种载体,可以快速构建质粒模板,实现体外的快速转录和翻译。该类优化的载体含有T7启动子,采用Xi-Clone定向克隆技术。
特点:
* 3种不同的配置选择:不标记表达、N-端HA融合标记和C-端HA融合标记 。采用G418选择稳定转染的细胞
* 利用anti-HA抗体可以快速检测表达蛋白
Orbigen develops, manufactures and markets reagent tools for proteomic research. We are best known for novel antibodies, innovative gene expression technologies, and reliable custom protein expression services. By being at the forefront of technology development, we are proud to provide timely and quality products that help you continuously push the limits of what's possible.
Orbigen is a young and rapidly growing company headquartered in San Diego, California, one of the world's largest clusters of life science research institutes and companies. We employ an extensive group of dynamic and talented scientists who are empowered to create unique and exciting solutions to address your research needs. Many of our scientists are founding members and seasoned veterans from industry leaders. Our experienced technical staff strives to provide high-quality products and expert technical support to life science researchers worldwide.
In addition to providing unique research tools we can also help you commercialize your ideas and reagents. We have extensive experience and proven track record for working with inventors to timely and effectively bring important reagent to market.