该技术具有定量功能并具有广泛的应用范围，VisEn公司的科学***在德***科隆举行的分子影像学会年会上展示了18篇摘要，包括在癌症药物测试、心血管疾病和骨骼再塑造领域的应用。
 VisEn医学公司总裁兼***席执行官Kirtland G.Poss说：“消费者对我们技术的认同接受度如此之高，我们感到十分兴奋，并打算扩大我们产品的商业运输以及使用。我们的产品是第***种能够在动物中真正进行定量、荧光活体三维成像的的光学系统。”，具有专利技术的成像系统和探针 被Massachusetts General Hospital , Harvard Medical School,及其它***研究机构广泛采用。
应用简述：肿瘤学、免疫性疾病如关节炎模型等、骨病学、肺病学、心血管病学等。
Quantitative Tomography, using FMT 2500 Imaging System, provides information-rich data that deepens the understanding of disease mechanisms and therapeutic responses. Based on proprietary FMT technology, this in vivo imaging system monitors and quantifies biological targets and pathways in real time.
● Non-invasive, whole body, deep tissue imaging under fluid-free conditions 
● Easily and efficiently obtain quantitative data in animal models  
● Measure and monitor multiple biological processes simultaneously 
● Co-register Quantitative Tomographic data with CT, PET or MR images 
● Compatible with near-infrared fluorescence agents from VisEn and other suppliers

Three Steps to Quantitative Data

STEP 1: FMT Data Generation 

Raster scan laser light to measure absorption profiles
Raster scan laser light to measure corresponding fluorescence profiles
Generate paired absorption and fluorescence data maps from10,000 -100,000 source-detector projections
STEP 2: FMT Normalization
Process all paired Absorption and Fluorescence acquisition data to generate normalized fluorescence measurements
Feed normalized data into FMT algorithmic models of photon transport in tissue
STEP 3: FMT Reconstruction

Generate fluorescence quantification at each point in the subject
Calculate fluorescence measurements throughout Regions of Interest
Feed data into readily compatible universal formats for analysis, data base building, and decision-making

Generate accurate, quantitative data
Mouse asthma model
Fluorescence agent: ProSense (cathepsin B activity)
Quantify disease levels and therapeutic response
Use reflectance mode for baseline comparison
Use Quantitative Tomography mode for 3D image and quantification  

技术规格：

System Imaging Parameters Two Imaging modes: Reflectance Imaging and Quantitative Tomography.
Available laser wavelengths:

Channel 1: Excitation = 670 nm, Emission = 700 nm
Channel 2: Excitation = 745 nm, Emission = 780 nm
FMT 2500 Software Within one integrated application, FMT 2500 Quantitative Tomography software provides:

system control
data acquisition
tomographic reconstruction
multiplexing capability
quantitative image analysis
data export and DICOM compatibility
Animal Handling Portable animal imaging cassette, multi-modality compatible

Integrated gas anesthesia capability
Heated internal animal imaging chamber
Optional Accessories Heated/anesthesia-ready external prep stations, linkable for increased throughput

Additional animal imaging cassettes
Multimodality adapters for CT, MR and PET co-registration 
Publications：
1、Oncology:
A spatially and temporally restricted mouse model of soft tissue sarcoma.
Kirsch D.G, Dinulescu D.M, Miller J.B, Grimm J, Santiago P.M, Young N.P, Nielsen G.P, Quade B.J, Chaber C.J, Schultz C.P, Takeuchi O, Bronson R.T, Crowley D, Korsmeyer S.J, Yoon S.S, Hornicek F.J, Weissleder R, Jacks T. Nature Medicine, Vol. 13 Issue 8, p992-997 (2007)
Colonic adenocarcinomas: near-infrared microcatheter imaging of smart probes for early detection--study in mice.
Alencar H , Funovics MA , Figueiredo J, Sawaya H , Weissleder R , Mahmood U . Radiology. Jul;244(1):232-8. Epub May 16 (2007)
Selective antitumor effect of novel protease-mediated photodynamic agent.
Choi Y, Weissleder R, Tung CH. Cancer Res. Jul 15;66(14):7225-9 (2006)
Use of gene expression profiling to direct in vivo molecular imaging of lung cancer.
Grimm J , Kirsch DG , Windsor SD , Kim CF , Santiago PM , Ntziachristos V , Jacks T , Weissleder R . Proc Natl Acad Sci USA. 102(40):14404-9 (2005).
Use of gene expression profiling to direct in vivo molecular imaging of lung cancer
Proc Natl Acad Sci USA , October (2005)
Tomographic fluorescence mapping of tumor targets.
Montet et al., Cancer Research Jul 15, 65 (14):6330-6, (2005)
Detection of Dysplastic Intestinal Adenomas Using Enzyme-Sensing Molecular Beacons in Mice
Gastroenterology, February (2002)
In vivo imaging of tumors with protease activated near-infrared fluorescent probes.
Nature Biotechnology, April (1999)
2、Inflammatory Disease：
In vivo imaging of protease activity in arthritis: a novel approach for monitoring treatment response. Wunder A , Tung CH , Muller-Ladner U , Weissleder R , Mahmood U . Arthritis & Rheumatism 50(8), 2459-2465 (2004).
3、Skeletal Disease：
Osteogenesis associates with inflammation in early-stage atherosclerosis evaluated by molecular imaging in vivo. Circulation. 2007 Dec 11;116(24):2841-50. Epub 2007 Nov 26.
Noninvasive optical detection of bone mineral. J Bone Miner Res. 2007 Aug;22(8):1208-16.
In vivo near-infrared fluorescence imaging of osteoblastic activity. Nature Biotechnology. 2001 Dec;19(12):1148-54.
4、Pulmonary Disease：
Haller JL, Hyde D, Deliolanis N , de Kleine R , Niedre M , Ntziachristos V . Visualization of Pulmonary Inflammation Using Non-invasive Fluorescence Molecular Imaging. J Appl Physiol., Jan (2008)
5、Cardiovascular Disease：
Dual channel optical tomographic imaging of leukocyte recruitment and protease activity in the healing myocardial infarct. Nahrendorf M , Sosnovik DE , Waterman P , Swirski FK , Pande AN , Aikawa E , Figueiredo JL , Pittet MJ , Weissleder R .
Circ Res. Apr 27;100(8):1218-25. Epub 2007 Mar 22 (2007)
Nanoparticle PET-CT Imaging of Macrophages in Inflammatory Atherosclerosis.
Matthias Nahrendorf , Hanwen Zhang, Sheena Hembrador, Peter Panizzi, David E. Sosnovik, Elena Aikawa, Peter Libby, Filip K. Swirski, and Ralph Weissleder. Circulation, Jan; 117: 379 – 387 (2008)
Molecular Imaging Identifies Proteolytic and Osteogenic Activities in Early Aortic Valve Disease.
Aikawa E, Nahrendorf M, Sosnovik D, Lok VM, Jaffer FA, Aikawa M, Weissleder R Multimodality Circulation.;115:377-386 (2007)
Inflammation in atherosclerosis: visualizing matrix metalloproteinase action in macrophages in vivo.
Deguchi JO , Aikawa M , Tung CH , Aikawa E ,Kim DE , Ntziachristos V , Weissleder R , Libby P. Circulation 114(1):55-62 (2006).
Near-infrared fluorescent imaging of matrix metalloproteinase activity after myocardial infarction.
Chen J , Tung CH , Allport JR , Chen S , Weissleder R , Huang PL . Circulation 111(14), 1800-1805 (2005).
In vivo imaging of proteolytic activity in atherosclerosis.
Chen J , Tung CH , Mahmood U , Ntziachristos V , Gyurko R , Fishman MC , Huang PL , Weissleder R . Circulation 105(23), 2766-2771 (2002).
In vivo Imaging of Proteolytic Activity in Atherosclerosis Circulation, June (2002)
 

内容